![]() Semi Dry Blotting has the added benefit of economic transfers due to very low buffer volumes - typically only a few millilitres of buffer are required per transfer. Each unit is compatible with their respective omniPAGE vertical gel system. All units can be used for all types of blotting: western, southern and northern via uncomplicated buffer and set up procedures and are compatible with gel thicknesses from 0.25 up to 10mm without the need for additional equipment. Power Blotter Pre-cut Membranes and Filters combined with Power Blotter 1-Step Transfer Buffer allow for high-efficiency semi-dry transfer of proteins in less than 10 minutes.These Semi Dry Blotters offer rapid transfer times for DNA, RNA and protein blotting typically 15 to 30 minutes. Invitrogen Power Blotter Pre-cut Membranes and Filters are convenient pre-cut membranes and filters that include 20 sheets of polyvinylidene difluoride (PVDF) or nitrocellulose and 80 sheets of absorbent blotting paper (0.85 mm thickness). The protein binding capacity is 209 µg/cm 2. The proteins bind to the membrane through hydrophobic and electrostatic interactions. ![]() The membrane is compatible with commonly used detection methods such as staining, chemiluminescence, and fluorescence. The nitrocellulose membrane (0.2 µm pore size) in the stack is composed of 100% pure nitrocellulose to provide high-quality transfer. This membrane has lower background levels and increased sensitivity for fluorescent probing than regular PVDF membranes. The proteins bind to the membrane through hydrophobic interactions. The membrane is compatible with commonly used detection methods such as staining, immunodetection, and fluorescence. The PVDF membrane (0.45 µm pore size, low fluorescence) has higher binding capacity than nitrocellulose. Each Power Blotter Select Transfer Stack contains filter paper, membrane, and the appropriate cathode and anode buffers incorporated into a solid gel matrix to allow fast, reliable transfer of proteins. The Power Blotter Select Transfer Stacks are pre-assembled stacks that come with an integrated preactivated PVDF or nitrocellulose transfer membrane for dry blotting of proteins from polyacrylamide gels. Images captured using automatic exposure on an iBright FL1000 Imaging System. Proteins were transferred in 7 minutes using a Power Blotter Select Transfer Stack (left, PB5310 PB3310) and Power Blotter Pre-cut Membranes and Filter stack (right, PB9320 PB7320), probed with target-specific primary antibodies and fluorescently conjugated secondary antibodies. ![]() Western blot analysis of several targets (KLH, EGFR, Hsp90, PDI and Cyclophilin B protein) was performed by loading serially diluted HeLa cell lysate with KLH spike (starting at 7.5 µg HeLa lysate, 7.5 µg KLH spike per well, serially diluted 2:3) onto Bolt 4-12% Bis-Tris Plus gels. Power Blotter Select Transfer Stacks and Power Blotter Pre-Cut Membrane and Filter stacks efficiently transfer high, mid, and low molecular weight proteins. ![]() Power Blotter with Pre-cut Membranes and Filters and Power Blotter 1-Step Transfer Buffer Power Blotter with Select Transfer Stacks ![]()
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